Vestnik of Orenburg State Pedagogical University. Electronic Scientific Journal. 2024. № 1 (49). P. 59—70

 

BIOLOGICAL SCIENCES

Original article

UDC 574.24

DOI: https://doi.org/10.32516/2303-9922.2024.49.5

Heavy metals content in Melilotus officinalis L. shoots during the introduction of soil microorganisms and humates into disturbed soil

Natalya A. Makeeva, Candidate of Biological Sciences, Researcher at the Laboratory of Land Reclamation and Biomonitoring
Federal Research Center of Coal and Coal Chemistry of the Siberian Branch of the Russian Academy of Sciences, Kemerovo, Russia, natykor@bk.ru, https://orcid.org/0000-0002-1971-4068

 

Abstract

This paper presents the results of an experiment to study the content of heavy metals in Melilotus officinalis L. shoots when soil microorganisms and potassium humate are introduced into the soil of the rock dump of the Kedrovsky coal mine. The soils of the studied area belong to medium loam, have an alkaline reaction of the medium (pH 7.8), the content of mobile forms of nitrogen is 1.7 mg/kg, phosphorus — 7 mg/kg and potassium — 125 mg/kg. The amount of heavy metals in the area where the experiment is laid does not exceed the MAC. The comparative analysis of the content of zinc, cadmium, lead, copper, iron, chromium and nickel in Melilotus officinalis L. shoots with vegetation on uncontaminated soils showed an insignificant amount of these elements. When microbiological preparations are introduced into the dump soil, the copper content decreases by 40—45%, iron — by 50—53%, lead — by 54—57%, chromium — by 66—79%, nickel — by 40—41% compared to the control. The amount of zinc when introducing soil microorganisms is 60% higher than the control, when introducing a mixture of soil microorganisms with potassium humate — by 150%.

Key words

Melilotus officinalis L., rock dump, coal mine, disturbed territories, reclamation, soil microorganisms, heavy metals.

Acknowledgments: The work was carried out according to the state task AAAA-A21-121011590010-5

For citation:

Makeeva N. A. Heavy metals content in Melilotus officinalis L. shoots during the introduction of soil microorganisms and humates into disturbed soil. Vestnik of Orenburg State Pedagogical University. Electronic Scientific Journal, 2024, no. 1 (49), pp. 59—70. DOI: https://doi.org/10.32516/2303-9922.2024.49.5.

The full text of the article PDF (Russian)

 

References

1. Artamonova V. S., Bortnikova S. B. Nekotorye aspekty ekologii rekul’tivatsii pochv na otvalakh vskryshnykh porod v raionakh ugledobychi [Ecological aspects of soil reclamation on overburden dumps in coal mining areas]. Antropogennaya transformatsiya prirodnoi sredy — Anthropogenic Transformation of Nature, 2022, vol. 8, no. 1, pp. 48—57. DOI: 10.17072/2410-8553-2022-1-48-57. (In Russian)
2. Artamonova V. S., Marchenko M. I. Sovremennye aspekty zhiznedeyatel’nosti molodykh pochv [Modern aspects of the life activity of young soils]. Prirodno-tekhnogennye kompleksy: rekul’tivatsiya i ustoichivoe funktsionirovanie: sb. materialov mezhdunar. nauch. konf. [Natural-technogenic complexes: reclamation and sustainable functioning. Proceed. of the Internat. sci. conf.]. Novosibirsk, Okarina Publ., 2013, pp. 57—59. (In Russian)
3. Babaeva T. M. Ochistka zagryaznennykh tyazhelymi metallami pochv metodom fitomelioratsii [Phyto-Melioration Cleaning of Soils Contaminated With Heavy Metals]. Byulleten’ nauki i praktiki — Bulletin of Science and Practice, 2019, vol. 5, no. 9, pp. 234—238. DOI: 10.33619/2414-2948/46/28. (In Russian)
4. Baraz V. R., Pegashkin V. F. Ispol’zovanie MS Excel dlya analiza statisticheskikh dannykh: ucheb. posobie [Using of MS Excel to analyze statistical data. Textbook]. Nizhnii Tagil, NTI (filial) UrFU Publ., 2014. 181 p. (In Russian)
5. Boguslavskii A. E., Androkhanov V. A., Kolmagorova Yu. O., Uzhogova A. A., Gossen I. N., Saeva O. P. Geokhimicheskii fon tyazhelykh metallov v pochvakh i rasteniyakh na uchastkakh otvalov ugol’nykh mestorozhdenii [Geochemical background of heavy metals in soils and plants in the sites of coal deposits]. Izvestiya Altaiskogo otdeleniya Russkogo geograficheskogo obshchestva — Bulletin of the Altay Branch of the Russian Geographical Society, 2021, no. 2 (61), pp. 40—50. DOI: 10.24412/2410-1192-2021-16104. (In Russian)
6. Vorob’eva E. E., Fotina N. V., Asyakina L. K., Osintseva M. A., Prosekov A. Yu. Otsenka khimicheskikh i sanitarnykh pokazatelei gruntov ugol’nykh otvalov yuga Kuznetskoi kotloviny [Chemical and sanitary assessment of coal spoil heaps in the south of the Kuznetsk Basin]. Transformatsiya ekosistem — Ecosystem Transformation, 2022, vol. 5, no. 4, pp. 83—97. DOI: 10.23859/estr-220603. DOI: 10.23859/estr-220603. (In Russian)
7. Geokhimiya okruzhayushchei sredy. Praktikum [Geochemistry of the environment. Practical guide]. St. Petersburg, RGGMU Publ., 2022. 48 p. (In Russian)
8. GN 2.1.7.2041-06. Predel’no dopustimye kontsentratsii (PDK) khimicheskikh veshchestv v pochve: Gigienicheskie normativy [GN 2.1.7.2041-06. Maximum allowable concentrations (MAC) of chemicals in soil: Hygienic standards]. Moscow, Federal’nyi tsentr gigieny i epidemiologii Rospotrebnadzora Publ., 2006. (In Russian)
9. Dedyu I. I. Ekologicheskii entsiklopedicheskii slovar’ [Ecological encyclopedic dictionary]. Kishinev, Gl. red. Mold. sovet. entsikl. Publ., 1989. 408 p. (In Russian)
10. Zherebtsov S. I., Ismagilov Z. R., Neverova O. A., Korniyasova N. A., Sokolov D. A. Guminovye veshchestva burykh uglei i perspektivy ikh primeneniya v rekul’tivatsii [Humic substances of brown-coals and prospects for their use in reclamation]. Razrabotka kompleksa tekhnologii rekul’tivatsii tekhnogenno narushennykh zemel’: materialy Vseros. nauch. konf. [Development of a complex of technologies for reclamation of disturbed lands. Proceed. of the All-Russia sci. conf.]. Kemerovo, 2011, pp. 20—23. (In Russian)
11. Zhuikova T. V., Bezel’ V. S. Ekologicheskaya toksikologiya: uchebnik i praktikum dlya bakalavriata i magistratury [Environmental toxicology. Textbook and workshop for undergraduate and graduate students]. Moscow, Yurait Publ., 2018. 362 p. (In Russian)
12. Zhumadilova Zh. Sh., Mukhambetov B., Abdieva K. M., Shorabaev E. Zh., Sadanov A. K. Vliyanie donnika na solevoi rezhim i organo-mineral’nyi sostav pochvy risovogo sevooborota v usloviyakh Priaral’ya [The influence of sweet clover on the salt regime and organo-mineral composition of the soil of rice crop rotation in the Aral Sea region]. Uspekhi sovremennogo estestvoznaniya — Advances in Current Natural Sciences, 2014, no. 12 (5), pp. 546—549. Available at: https://natural-sciences.ru/ru/article/view?id=34661. (In Russian)
13. Zubova L. G., Zubov A. R., Verekh-Belousova K. I., Oleinik N. V. Poluchenie metallov iz terrikonov ugol’nykh shakht Donbassa [Obtaining metals from waste heaps of Donbass coal mines]. Lugansk, VNU im. V. Dalya Publ., 2012. 144 p. (In Russian)
14. Il’in V. B. Tyazhelye metally v sisteme pochva — rastenie [Heavy metals in the soil-plant system]. Novosibirsk, Nauka Publ., 1991. 151 p. (In Russian)
15. Kovalevskii A. V., Luchnikova E. M., Astaf’eva M. V., Filippova A. V. Soderzhanie tyazhelykh metallov v plodakh zemlyaniki lesnoi Fragaria vesca L. na rekul’tivirovannykh otvalakh ugol’nykh predpriyatii Kuzbassa [The content of heavy metals in the fruits of wild strawberry Fragaria vesca L. on reclaimed dumps of coal enterprises in Kuzbass]. Problemy i perspektivy sovremennoi nauchnoi mysli v Rossii i za rubezhom: sb. tez. III Mezhdunar. konf. [Problems and prospects of modern scientific thought in Russia and abroad. Abstr. of the III Internat. conf.]. Kemerovo, 2021, pp. 31—33. (In Russian)
16. Kokoev V. T., Yakovlev S. N. Zagryaznenie pochv tyazhelymi metallami v raione gornodobyvayushchikh ob’’ektov (na primere Kvaisinskogo rudnika) [Soil contamination with heavy metals in the area of mining facilities (using the example of the Kvaisinsky mine)]. Gornyi informatsionno-analiticheskii byulleten’ — Mining Informational and Analytical Bulletin, 2005, no. 5, pp. 318—319. (In Russian)
17. Kostenkov N. M., Oznobikhin V. I. Biologicheskaya rekul’tivatsiya porod ugol’nykh otvalov [Biological reclamation of coal dump rocks]. Vladivostok, Dal’nauka Publ., 2007. 99 p. (In Russian)
18. Kupriyanov A. N., Manakov Yu. A., Barannik L. P. Vosstanovlenie ekosistem na otvalakh gornodobyvayushchei promyshlennosti Kuzbassa [Restoration of ecosystems on dumps of the Kuzbass mining industry]. Novosibirsk, Geo Publ., 2010. 160 p. (In Russian)
19. Kurachev V. M., Androkhanov V. A. Klassifikatsiya pochv tekhnogennykh landshaftov [Classification of Soils of Technogenous Landscapes]. Sibirskii ekologicheskii zhurnal — Contemporary Problems of Ecology, 2002, no. 3, pp. 255—261. (In Russian)
20. Lamanova T. G., Sheremet N. V. Agrofitotsenozy na otvalakh v yuzhnoi chasti Kuznetskoi kotloviny [Agrophytocenoses on dumps in the southern part of the Kuznetsk Basin]. Novosibirsk, ZAO IPP “OFSET” Publ., 2010. 226 p. (In Russian)
21. Levkin N. D., Mukhina N. E. Vliyanie porodnykh otvalov ugol’nykh shakht na sostoyanie okruzhayushchei sredy [Impact of coal mine waste dumps on the environment]. Aktual’nye problemy gumanitarnykh i estestvennykh nauk, 2011, no. 5, pp. 277—279. (In Russian)
22. Makeeva N. A. Vliyanie inokulyata pochvennykh mikroorganizmov na agrokhimicheskie pokazateli tekhnogennykh elyuviev i produktsionnye protsessy Avena sativa L. v usloviyakh porodnogo otvala: dis. … kand. biol. nauk [The influence of the inoculum of soil microorganisms on the agrochemical parameters of technogenic eluvium and the production processes of Avena sativa L. in the conditions of a rock dump. Cand. Dis.]. Novosibirsk, 2015. 161 p. (In Russian)
23. Makeeva N. A. Izuchenie vliyaniya gumatov natriya na dinamiku rosta ovsa v usloviyakh porodnogo otvala ugol’nogo razreza [Study of the effect of sodium humate on growth of oats in the conditions of the dump coal mine]. Sovremennye problemy nauki i obrazovaniya — Modern Problems of Science and Education, 2014, no. 6. Available at: https://science-education.ru/ru/article/view?id=16673. (In Russian)
24. Makeeva N. A., Neverova O. A. Biogennost’ porodnogo otvala pri vnesenii pochvennykh mikroorganizmov [Biogenic of waste dump at entering soil microorganisms]. Sovremennye problemy nauki i obrazovaniya — Modern Problems of Science and Education, 2015, no. 6. Available at: https://science-education.ru/ru/article/view?id=23216. (In Russian)
25. Makeeva N. A., Neverova O. A. Dinamika biologicheskoi aktivnosti embriozemov v prikronovoi zone drevesnykh rastenii na otvalakh ugol’nykh razrezov [The dynamics of biological activity in technosols around crown perimeters of woody plants on coal mine waste piles]. Trudy Karel’skogo nauchnogo tsentra Rossiiskoi akademii nauk — Transactions of Karelian Research Centre of Russian Academy of Science, 2022, no. 3, pp. 42—52. DOI: 10.17076/eco1516. (In Russian)
26. Metodicheskie ukazaniya po opredeleniyu tyazhelykh metallov v pochvakh sel’khozugodii i produktsii rastenievodstva [Guidelines for the determination of heavy metals in soils of farmland and crop products]. Moscow, TsINAO Publ., 1992. 65 p. (In Russian)
27. Sadanov A. K., Kurmanbaev A. A. Ekologicheskaya tekhnologiya v biologizatsii zemledeliya [Ecological technology in biologization of agriculture]. Almaty, Agrouniversitet Publ., 1999. 48 p. (In Russian)
28. Semenova I. N., Sundukov Ya. T., Il’bulova G. R. Biologicheskaya aktivnost’ pochv kak indikator ikh ekologicheskogo sostoyaniya v usloviyakh tekhnogennogo zagryazneniya tyazhelymi metallami [Biological activity of soils as an indicator of their ecological state in conditions of technogenic pollution with heavy metals]. Ufa, Gilem Publ., 2012. 196 p. (In Russian)
29. Titov V. N., Mamonov A. N. Perspektivy ispol’zovaniya razlichnykh vidov donnika i fatselii v kachestve fitomeliorantov v usloviyakh Saratovskoi oblasti [Prospects for the use of various types of sweet clover and phacelia as phytomeliorants in the Saratov region]. Vestnik Orlovskogo gosudarstvennogo agrarnogo universiteta, 2011, vol. 29, no. 2, pp. 15—17. (In Russian)
30. Tkacheva E. V., Galkina M. A., Stogova A. V. Terrikony v g. Safonovo Smolenskoi oblasti: rastitel’nyi pokrov 20 let spustya [Spoil heaps in Safonovo, Smolensk region: changes in vegetation cover 20 years later]. Transformatsiya ekosistem — Ecosystem Transformation, 2021, no. 4 (4), pp. 54—64. DOI: 10.23859/estr-210625. (In Russian)
31. Fedotova A. S. Soderzhanie tyazhelykh metallov v otvalakh, obrazovannykh vskryshnymi porodami na ugol’nykh razrezakh [Content of heavy metals in dumps formed by overburden rocks in coal mines]. Gornyi informatsionno-analiticheskii byulleten’ — Mining Informational and Analytical Bulletin, 2006, no. 1, pp. 200—205. (In Russian)
32. Frolova A. S., Pereverzeva M. K., Asyakina L. K., Golubtsova Yu. V., Osintseva M. A. Fermentativnaya aktivnost’ tekhnogennykh poverkhnostnykh obrazovanii Kuzbassa [Enzymative activity of technogenic surface formations of Kuzbass]. Agrarnaya nauka Evro-Severo-Vostoka — Agricultural Science Euro-North-East, 2022, no. 23 (4), pp. 538—547. DOI: 10.30766/2072-9081.2022.23.4.538-547. (In Russian)
33. Khasanova Ya. T., Suyundukov G. Ya., Biktimerova M. B., Suyundukova M. B. Ekologo-toksikologicheskoe sostoyanie pochv agroekosistem v zone vliyaniya gornorudnykh predpriyatii [Ecological and toxicological state of soils of agroecosystems in the zone of influence of mining enterprises]. Biologicheskie sistemy: ustoichivost’, printsipy i mekhanizmy funktsionirovaniya: sb. statei V Vseros. nauch.-prakt. konf. [Biological systems: stability, principles and mechanisms of functioning. Proceed. of the V All-Russia sci.-pract. conf.]. Nizhnii Tagil, 2017, pp. 330—335. (In Russian)
34. Sheremetov R. T. Osobennosti snezhnogo pokrova v period maksimuma snegonakopleniya 2006 g. na otvalakh Kedrovskogo razreza [Features of snow cover during the period of maximum snow accumulation in 2006 on the dumps of the Kedrovsky open-pit mine]. Rekul’tivatsiya narushennykh zemel’ v Sibiri: sb. nauch. tr. [Recultivation of disturbed lands in Siberia. Collect. sci. papers]. Kemerovo, Irbis Publ., 2009, pp. 35—39. (In Russian)
35. Ekologicheskaya karta Kemerovskoi oblasti. Masshtab 1:500000 [Ecological map of the Kemerovo region. Scale 1:500000]. Moscow, Federal’naya sluzhba geodezii i kartografii Rossii Publ., 1995. 1 sh. (In Russian)
36. Gadzhiev I. M., Kurachev V. M., Ragim-Zade F. K. (comp.) Ekologiya i rekul’tivatsiya tekhnogennykh landshaftov [Ecology and reclamation of technogenic landscapes]. Novosibirsk, Nauka. Sib. otd-nie Publ., 1992. 305 p. (In Russian)
37. Dang Z., Liu C., Haigh M. J. Mobility of heavy metals associated with the natural weathering of coal mine spoils. Environmental Pollution, 2002, no. 3, pp. 419—426.
38. Egorova I. N., Neverova O. A. Analysis of distribution of radionuclides in various organs of Taraxacum officinale Web., which grows in rock waste disposal areas of coal pits in Kuznetsk basin. World Applied Sciences Journal, 2013, vol. 24, no. 3, pp. 345—349. DOI: 10.5829/idosi.wasj.2013.24.03.13202.
39. Ismagilov Z. R., Zhuravleva N. V., Kerzhentsev M. A., Yashnik S. A., Matus E. V., Podyacheva O. Yu., Khairulin S., Shikina N. V., Ismagilov Z., Kozlov A. P., Smirnov V. G. Environmental issues in Kuznetsk coal basin. Scientific approaches and technologies to reduce environmental pollution. Chemistry for Sustainable Development, 2018, vol. 26, no. 3, pp. 221—239. DOI: 10.15372/CSD20180302.
40. Liu Xiaoyang, Bai Z., Shi H., Zhou W., Liu Xiaocai. Heavy metal pollution of soils from coal mines in China. Natural Hazards, 2019, vol. 99, pp. 1163—1177. DOI: 10.1007/s11069-019-03771-5.
41. Makeeva N., Neverova O. The influence of soil microorganisms on heavy metal content in the substrate of waste rock dumps. VI International Scientific Conference “Problems of Industrial Botany of Industrially Developed Regions”. Kemerovo, 2021. DOI: 10.1051/bioconf/20213100017.
42. Zahra N., Kalim I. Perilous effects of heavy metals contamination on human health. Pakistan Journal of Analytical & Environmental Chemistry, 2017, vol. 18, no. 1, pp. 1—17. DOI: 10.21743/pjaec/2017.06.01.